REVIEW Open Access [601347]

REVIEW Open Access
Innovative strategies for the management
of long bone infection: a review of the
Masquelet technique
Vivek Chadayammuri1, Mark Hake2and Cyril Mauffrey3*
Abstract
Post-traumatic long bone osteomyelitis (PTOM) is a relatively frequent occurrence in patients with severe open
fractures and requires treatment to prevent limb-threatening complications. The Masquelet technique represents a
length-independent, two-staged reconstruction that involves the induction of a periosteal membrane and use of an
antibiotic-impregnated cement spacer for the treatment of segmental bone loss that result from bone infection. In
this review, we summarize recent developments regarding the diagnosis and treatment of long bone PTOM, with a
special emphasis on the use of the Masquelet technique for reconstruction of wide diaphyseal defects.
Keywords: Osteomyelitis, Posttraumatic, Defects, Diaphyseal, Reconstruction, Masquelet, Antibiotic, ORIF, Fixation
Introduction
Osteomyelitis, or infection of the bone, represents a
complex and challenging clinical entity in the field of or-
thopedics. In 1951, Gallie et al reported a case of recurring
osteomyelitis following a period of 80 years since onset of
initial infection. The patient was a 90-year-old woman
with a Brodie ’s abscess localized to the distal femur. Given
a largely asymptomatic presentation throughout the
patient ’s lifetime, diagnosis and treatment was exceedingly
delayed. This case is but one of many that illustrates the
complex nature of osteomyelitis [1].
The focus of this review will be on long bone posttrau-
matic osteomyelitis (PTOM), defined as infection of the
bone in conjunction with recent fracture or traumatic
insult. Long bone PTOM is a relatively frequent occur-
rence and may be involved in as many as 10 % of all open
fractures and 1 % of all closed fractures [2]. Several etio-
logical factors have been previously described, including
direct inoculation at time of injury, macro- or microvascu-
lar damage, surgical contamination, host immunodefi-
ciency, and/or postoperative wound contamination [3 –5].
Barring early diagnosis and adequate treatment, long bonePTOM may result in fracture nonunion, sepsis, and dif-
fuse tissue devitalization underlying a requirement for
limb amputation [4].
Diagnostic evaluation of long bone PTOM
The clinical diagnosis of long bone PTOM is challenging,
in large measure, owing to the non-specific nature of its
initial presentation. In addition to findings of localized
pain, long bone PTOM classically presents with signs and
symptoms of infection includi ng low-grade fever, erythema,
edema, and/or draining sinus tracts [5, 6]. In pediatric pa-
tients, presentation may also include systemic manifesta-
tions such as fever, chills, an d night sweats [5]. Clinical
examination should reveal loca lized tenderness to palpation
overlying an aspect of bone with prior or current fracture.
D i a g n o s t i cw o r k – u po fl o n gb o n eP T O Mt r a d i t i o n –
ally involves a combination of imaging, tissue culture,
and laboratory studies [7]. Acute inflammatory
markers such as erythrocyte sedimentation rate (ESR),
C-reactive protein (CRP), and leukocyte count (WBC)
have low specificity for diagnosis, particularly in the
setting of a profound systemic inflammatory response
(e.g. rheumatoid arthritis, Crohn ’s disease, systemic
lupus erythematosus, etc.). Therefore, trending levels
of acute inflammatory markers is more appropriately
reserved for monitoring infection status following ini-
tiation of treatment [5, 6]. The ‘gold standard ’for the* Correspondence: Cyril.mauffrey@dhha.org
3Department of Orthopaedic Surgery, Denver Health Medical Center,
University of Colorado, School of Medicine, 777 Bannock Street, Denver, CO
80204, USA
Full list of author information is available at the end of the article
© 2015 Chadayammuri et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Chadayammuri et al. Patient Safety in Surgery (2015) 9:32
DOI 10.1186/s13037-015-0079-0

diagnosis of PTOM is a bone culture obtained in the oper-
ating room; however, this should always be complemented
by histopathological analysis to reduce the incidence of
false-positives [7, 8]. Currently ongoing clinical studies are
investigating newer technologies such as polymerase chain
reaction (PCR) and fluorescence in situ hybridization
(FISH) that may improve diagnostic sensitivity; however,
additional research is required to inform the feasibility
and validity of such modalities [8, 9].
Following positive findings on culture and pathology
suggestive of long bone PTOM, preoperative work-up
must include an imaging series consisting of anteroposter-
ior (AP) and lateral radiography, MRI, and/or CT. A
multimodal imaging approach is employed to surmount
the independent limitations of each imaging modality. In
patients with long bone PTOM, AP and lateral radio-
graphs traditionally demonstrate regional osteopenia, peri-
osteal reaction ( “Codman ’s triangle ”), and sequestrum
(segments of necrotic bone with interspersed viable granu-
lation tissue). However, sensitivity of plain radiography is
quite poor during the initial two weeks of infection [5].
Computed tomography (CT) may enable earlier detection
of infection through visualization of devitalized cortical
bone, sequestrum, and/or involucrum (periosteal bone
formation) on multiplanar reconstructions but demon-
strates poor capacity to delineate soft-tissue involvement
[10] .Magnetic resonance imaging (MRI) enables excellent
visualization of soft-tissue pathology in as early as 3 –5
days following initial onset of long bone PTOM [10] and
is therefore considered the ‘gold standard ’for confirm-
ation of osteomyelitis infection via imaging studies. Exud-
ate, edema, or sequestrum appear as hypodense lesions on
T1-weighted MRI images and short-tau inversion recovery
(STIR) sequences, while surrounding granulation tissue
appears as a low-intensity signal on T1-weighted images
and high-intensity signal on STIR sequences or T2-
weighted imaging [10]. The downside of MRI use is the
artifact in the presence of hardware.
The classification of long bone PTOM is done according
duration (Waldvogel et al) or disease stage (Cierny-Mader
et al) and may facilitate treatment planning [11]. In the
Walvogel classification, osteomyelitis is classified on the
basis of being hematogenous, contiguous, or chronic in
nature. In the Cierny-Mader classification, osteomyelitis is
categorized by anatomic location into Stage 1 (medullary),
Stage 2 (superficial), Stage 3 (localized), and Stage 4 (dif-
fuse). This scheme also incorporates consideration of the
host ’s health status, divided into local factors (chronic
lymphedema, venous stasis, or arteritis) or systemic
factors (malnutrition, renal failure, diabetes mellitus, and
immunodeficiency status). Both classification systems can
be useful for informing diagnosis and treatment of long
bone PTOM; however, a critical shortcoming is that ana-
tomical staging within these classification schemes is oftenconfounded by the presence of orthopedic hardware that
generates artifact interference on imaging. In addition, no
classification schemes incorporate the location of the
infection on long bones (intra articular, metaphyseal or
diaphyseal), which in our view is an important consider-
ation for the treatment plan.
Pre-operative optimization of the patient
The management of long bone PTOM is complex and
challenging. Therefore, patients must be prepared for a
long course of multiple surgeries and counseled on the
risks for postoperative complications that include non-
union, hardware failure and infection recurrence. More-
over, initial stages of preoperative planning must involve
correction of modifiable co-morbidities and/or risk factors
that portend poor postoperative wound healing. In-
deed, a study by Brinkler et al showed that 31 of 37
patients (83 %) with fracture nonunion had one or
more underlying metabolic or endocrine abnormalities
such as vitamin D deficiency, calcium imbalances,
central hypogonadism, thyroid disorders, and parathyroid
hormone disorders. Eight of these patients (25 %) achieved
bony union in an average of 7.6 months (range, 3 to 12
months) following treatment of their metabolic or endo-
crine abnormalities without further operative treatment
[12]. In addition to nutritional and metabolic testing, pa-
tients with long bone PTOM should also be evaluated for
poor glycemic control (in diabetic patients), tobacco or
illicit drug use, malnutrition, and vascular insufficiency of
the affected limb [13]. Low socioeconomic status (SES) is
also a prognostic indicator of worse treatment outcome
[14], a fact that should not deter the provision of care but
rather alert the treating surgeon to the increased potential
for postoperative complications.
Surgical treatment options
In severe cases of long bone PTOM, debridement of the
infected tissue results in extended diaphyseal loss of
bone that cannot be adequately managed by conven-
tional methods of reconstruction. In particular, conven-
tional methods often fail to satisfy at least one of the
following goals of therapy:
I. Control of the local infection with radical
debridement and antibiotic therapy
II. Fracture stabilization when instability occurs due to
debridement or nonunion
III.Provision of adequate soft-tissue coverage to ensure
wound healing [ 2,5]
Radical debridement may further precipitate widening
of the osseous defect in cases where segmental bone de-
fects exceed 5 cm in size [15]. Conventional techniques
such as vascularized fibula autograft and Iliazarov boneChadayammuri et al. Patient Safety in Surgery (2015) 9:32 Page 2 of 10

transport also yield poor long-term outcomes, often due
to graft resorption and revascularization by creeping
substitution [16, 17]. These treatment options are tech-
nically demanding and typically are not performed with-
out specialized training. Bone regeneration may also be
impeded secondary to inadequate vascularization or
soft-tissue coverage [18]. Finally, insufficient delivery of
concentrated antibiotic therapy to the site of infection
may result in high rates of disease recurrence.
The Masquelet technique represents a two-staged re-
constructive procedure that overcomes several of the
shortcomings in the treatment of osteomyelitis defects,
particularly those located to the long bone and associ-
ated with infected and/or no n-viable soft tissue [19].
First developed in the late 1970 ’sb yA CM a s q u e l e tb u t
only recently popularized, the chief advantages of this
strategy include control of the local infection with rad-
ical debridement, placemen t of a polymethylmethacry-
late (PMMA) cement spacer for maintenance of dead
space, and induction of a periosteal membrane that
protects against graft resorption. Furthermore, the
Masquelet technique is length-independent and is
therefore a viable option for the treatment of larger os-
seous defects. A detailed description of the Masquelet
technique with ‘Tips & Tricks ’and an illustrative case
example are provided in the following sections.
Two-staged reconstruction of extended
diaphyseal bone defects using the masequelet
technique
Stage 1
A. Radical debridement
The Masquelet technique is performed in two stages. In
the setting of an unstable long bone with infected and/
or non-viable soft tissue, the first stage involves radical
debridement of all infected or non-viable bone and in-
terposed fibrous tissue. Given that devitalized tissue
serves as a nidus for recurrent infection and predisposes
to increased risk of postoperative complications such as
delayed union, nonunion, and vascular thrombosis, re-
construction of the bone defect (conducted in Stage 2) is
only possible once complete eradication of infected and
non-viable tissues has been achieved. The margins of
debridement should extend until viable bony edges are
encountered, determined intraoperatively using the
“paprika sign ”(punctate bleeding upon drilling with a
2.5 mm drill bit). Once the margins of debridement have
been determined, an osteotome can be utilized to per-
form a corticotomy in order to prevent destruction of
healthy surrounding tissues.
B. Limb stabilization
Following debridement, stabilization must be achieved
to maintain length, alignment and rotation prior toinsertion of the cement spacer. Options include
unilateral or ring external fi xation, plate osteosynth-
esis, or IM nailing. The choice of stabilization is
based on the location of the defect. For bone loss in
the mid-diaphysis, an IM nail offers stable fixation
that allows early weight bearing. A narrow diameter
nail is used and coated with antibiotic-impregnated
cement. For defects close to an articular surface, ex-
ternal fixation is preferred. Ring-fixators offer stable
fixation and the ability to modify the bony alignment
postoperatively. When placing external fixation, care
must be taken to keep pins away from the site of de-
finitive fixation so that the external fixator can be left
in place until healing is achieved (during Stage 2 of
the procedure) [18].
In our experience, the preparation of an antibiotic
IM interlocking carbon-fiber nail can be achieved
using a simple and reproducible technique [20]. Plas-
tic tubing such as chest tubes, D&C tubing and D&E
tubing are used as a mold. The tubing is cut to a
length such that the proximal threaded portion of the
nail is left free of cement. The inner portion of the
tube is coated with sterile mineral oil to facilitate ex-
traction of the nail. One end of the tubing is then
clamped with a Kocher while the other end is loaded
with the viscous cement-antibiotic preparation using a
cement gun. The authors use 3 g of Vancomycin
powder combined with 40 g of Palacos-R (Zimmer,
Warsaw, Indiana) PMMA cement. When polymicro-
bial of gram-negative infection is suspected or dem-
onstrated by bone cultures, 3.6 g of Tobramycin can
be added to the mix. An extra 10 to 20 cc of mono-
mer should be used to obtain injectable cement, and
mixing should be performed under a vacuum to im-
prove antibiotic elution profile [21]. An IM nail is
then inserted centrally into the chest tube to produce
a wide cement mantle with a consistent thickness.
The entire construct is then placed into a cool sterile
saline bath during the exothermic polymerization
process to prevent melting of the inner layer of the
plastic tube and facilitate removal of the nail [22].
The authors recommend allowing the interlocking
holes to be covered with cement. Once hardened, the
chest tube is cut longitudinally and peeled off of the
cement-coated nail. The distal end of the cement
mantle can be contoured with a rasp to allow for
easier insertion. The intramedullary canal is then pre-
pared in a standard fashion for nail insertion. The
cement-coated nail is often wider than standard nails
so aggressive reaming may be necessary prior to
placement. If there is concern for proximal or distal
spread of the infection in the canal, a Reamer-
Irrigator-Aspirator (RIA) system may be used. The
IM nail is then inserted and statically locked underChadayammuri et al. Patient Safety in Surgery (2015) 9:32 Page 3 of 10

fluoroscopic guidance, with proper alignment con-
firmed by plain radiography.
C. Placement of the antibiotic-impregnated cement spacer
The next step following bony stabilization is the fash-
ioning of a cement spacer, traditionally composed of
polymethyl methacrylate (PMMA) cement, to fill the
segmental bone defect. A cement spacer is preferable
over other modalities of local antibiotic delivery pri-
marily because it assumes the conjoint functions of
inhibiting fibrous tissue ingrowth and maintaining
dead space volume until time of reconstruction [15].
For optimal outcome, the cement spacer should fill
the intramedullary canal and edges of surrounding vi-
able bone [18]. It is important to use a spacer to fill
the entire defect as opposed to antibiotic cement
beads. A membrane will form around the cement,
which will be filled with bone graft during the second
stage of the procedure. Beads leave an irregular mem-
brane that is less than ideal for containment of the
graft. Furthermore, the authors recommend irrigating
with cool saline during the exothermic polymerization
process to prevent local tissue necrosis. The cement
spacer may also be premixed with antibiotic to enable
localized delivery of higher concentrations than would
be feasible with systemic therapy. This practice also
provides a convenient and controlled dosing scheme
that relinquishes issues related to poor patient com-
pliance [23, 24].
The appropriate choice of antibiotic therapy is predi-
cated on the results of culture and pathology testing per-
formed on direct wound and bone samples. Additionally,
the chosen antibiotic must be thermostable to the exo-
thermic polymerization (solidification) process of the
PMMA cement. Aminoglycosides (gentamicin, tobra-
mycin) and vancomycin represent good options given
their thermostability, broad-spectrum of activity, high
rates of elution, and relatively low incidence of anaphyl-
actic reactions [25, 26]. In an in vitro study by Chang et
al, the longest duration of antibiotic-elution from
PMMA cement spacers was observed with gentamicin
as compared to vancomycin, teicoplanin, ceftazidime,
imipenem, piperacillin, or tobramycin [27]. In this study,
gentamicin also demonstrated excellent coverage against
methicillin-sensitive Staphylococcus aureus , coagulase
negative Staphylococci, Pseudomonas aeruginosa and
Escherichia coli species. In another study performed in
15 mongrel dogs, Adams et al compared the elution
characteristics of PMMA spacers loaded with cefazolin
(Ancef; 4.5 g/40 g cement powder), ciprofloxacin (Cipro;
6 g/40 g powder), clindamycin (Cleocin; 6 g/40 g pow-
der), ticarcillin (Ticar; 12 g/40 g powder), tobramycin
(Nebcin; 9.8 g/40 g powder), and vancomycin (Vancocin;
4 g/40 g powder). Clindamycin proved to have the bestelution profile in seromas, granulation tissue, and bone.
High concentrations of tobramycin were observed accu-
mulating in bone and granulation tissue, compared to
high concentrations of vancomycin occurring in bone
alone [28]. In an in vitro study by Penner et al, the
elution of tobramycin and vancomycin from Palacos-R
cement was found to persist over the course of 9 weeks
[29]. This study also demonstrated that the dual admin-
istration of these two antibiotics increased antibiotic
elution by 68 % for tobramycin and 103 % for vancomycin
as compared to the use of each antibiotic independently.
Finally, Wahlig et al. determined local concentrations of
gentamycin to be as high as 80 mg/mL after 4 days post-
implantation in a series of 41 patients treated with
gentamycin impregnated PMMA beads [30].
Of note, the largest permissible ratio is 8 g of
antibiotic per 40 g of cement ;higher doses of antibiotic
may impede cement molding [31]. During placement of
the cement block it is critical to irrigate the spacer with
cold saline during its polymerization phase as the raised
temperature may cause skin burn.
D. Soft-tissue coverage and wound healing
In the final phase of stage 1, there must be closure of
the wound without tension. This may require treatments
ranging from wet-to-dry dressings to a flap procedure to
provide adequate soft-tissue coverage. As a guiding
principle, the least technically demanding strategy that
enables successful soft-tissue coverage should be chosen.
For acute injuries, free-flaps are preferred over rotational
muscle flaps, as the latter can potentially increase
destruction of viable surrounding tissue [14]. In a pro-
spective study of 11 patients undergoing reconstruction
of diaphyseal defects averaging 10.5 cm (range, 5 to 18
cm), 6 patients (54.5 %) required soft-tissue repair by
flaps (3 free flaps, 3 pedicled muscle flaps). At 24 month
follow-up, all flaps were viable and there was no recur-
rence of infection [19]. In Masquelet ’s first report of the
technique, 28 of 35 patients (80 %) undergoing recon-
struction for long bone segmental defects (range, 5 to 24
cm) required soft-tissue repair procedures (14 free-flap,
14 pedicled muscle flap). Thirty-one patients (89 %)
were able to resume unprotected weight-bearing at a
mean of 8.5 months (range, 6 to 17 months). Four pa-
tients (11 %) sustained late stress fractures and required
further cast immobilization to achieve complete healing.
There were no cases of infection recurrence, which the
authors attribute to aggressive initial debridement [19].
Wound vacuum-assisted closure (VAC) can also be
used to promote tissue granulation, reduce tension re-
quired for wound closure, an d minimize postoperative
complications [32]. This is believed to occur second-
ary to a variety of mechanisms: (1) increased endothe-
lial proliferation and angiogenesis, (2) increasedChadayammuri et al. Patient Safety in Surgery (2015) 9:32 Page 4 of 10

tensile force that promotes tissue granulation and ac-
celerated wound closure, and (3) presence of an air-
tight negative-pressure seal that reduces interstitial
edema [33]. While data on clinical outcomes
following use of wound VAC therapy in patients with
osteomyelitis remains limited, one retrospective study
found that patients with osteomyelitis treated by
wound VAC experienced significantly lower rates of
infection recurrence and required less flap procedures
relative to patients treated by conventional wound
management strategies. Debridement time and type
was similar between the two groups [34]. In a separ-
ate study of 20 pigs with open fractures treated by
antibiotic-impregnated PMMA beads containing
vancomycin and tobramycin, concentrations of locally
eluted antibiotics were unaltered by the application of
wound VAC therapy [35].
On a final note, a randomized control trial performed
by Bouachour et al demonstrated potential benefit with
treatment by hyperbaric oxygen therapy (HBO) in pa-
tients suffering severe crush injuries of the limbs [36].
The study involved 36 patients with severe (grade III)
crush injuries who were randomly allocated into HBO
or placebo treatment groups within 24 h of surgical re-
construction. Compared to the placebo group, patients
receiving HBO had significantly lower requirements for
additional surgical procedures (flaps, grafts, vascular sur-
gery, or amputation); moreover, complete wound healing
was achieved in 87.5 % of patients receiving HBO com-
pared to only 30 % of patients receiving placebo. Hence,
augmentation of surgical reconstruction of severe crush
injuries with HBO may improve clinical outcome; how-
ever, additional studies are required to inform this treat-
ment strategy.
Following completion of stage 1 of the Masquelet
procedure, weight-bearing is determined based upon
the stability of the defect size, location and implant.
Patients with small and medium diaphyseal defects
treated by IM nailing can bear weight as tolerated.
The patient is then placed on a prolonged systemic
antibiotic regimen for a period of 6-8 weeks. This is
done in order to allow adequate time for a number
of processes to occur: (1) epithelialization of free or pedi-
cled muscle flaps in order to prevent surgical site contam-
ination by bacterial skin flora, (2) revascularization of
marginally viable tissue surrounding the bony defect, (3)
formation of the self-induced periosteal membrane, and
(4) treatment of any residual infection by systemic and/or
local antibiotics [19, 23, 37].
Stage 2
E. Clearance of infection
Complete eradication of infection is a prerequisite to re-
construction of bone defects due to osteomyelitis (Stage2 of the Masquelet technique). Trending inflammatory
markers before and after completion of systemic antibi-
otics can help confirm clearance. If there remains any
doubt as to the presence of residual infection, then tis-
sue specimens at the site of the segmental defect can be
harvested for culture and pathology [18]. Sending sam-
ples for pathology is critical due to the high rate of false-
negative culture results [8]. Levels of acute inflammatory
markers, including CRP and ESR, should be normal in
patients lacking comorbidities [15]. In the second stage
of reconstruction, the cement spacer is carefully re-
moved and the resulting cavity is filled with morcelized
autogenous corticocancellous bone graft.
F. Removal of the cement spacer and permanent fixation of
the fracture
A single longitudinal incision is made centrally through
the self-induced periosteal membrane. The cement spa-
cer should be removed in one piece or a few small pieces
created with a saw or osteotome. Particular care must be
taken to avoid iatrogenic injury to the induced periosteal
membrane so that it remains a self-contained compart-
ment. The ends of the resected bone margins should be
freshened with a drill bit or rasp to remove sclerotic
bone and facilitate bone graft integration. The medullary
canal should also be debrided to enable communicate
with the graft. Definitive fixation can be revised at this
point if necessary.
H. Harvest of autogenous bone graft using the Reamer/
Irrigator/Aspirator (RIA) system
The hollowed periosteal cavity is best filled with morce-
lized autogenous bone graft. A synergistic effect between
the bone graft and the induced membrane promotes in-
creased bone formation, angiogenesis, and consolidation
of the bony defect through stimulating the release of
growth factors such as VEGF, TGF-beta 1, and BMP-2
[19, 38 –40].
Bone graft can be harvested from a number of loca-
tions, including the iliac crest, proximal tibia and calca-
neus. Use of the RIA system from the femur is preferred
and portends less morbidity than iliac crest bone graft
harvesting [41 –43]. Furthermore, RIA aspirate has been
shown to contain osteoprogenitor cells and tissue
growth-factors (BMP-2, FGF-2, IGF-1, and TGF- β) that
may accelerate bone repair [44, 45]. In a prospective
study of 10 subjects, Sagi et al determined that aspirate
obtained from medullary canal of the femur via RIA
contained significantly higher levels of osteoinductive
compounds compared to conventional iliac crest bone
graft harvests [45]. A cadaveric study by Kovar et al fur-
ther determined a significantly greater quantity of bone
graft to be harvestable from the medullary canal of the
femur compared to the tibia using RIA reaming [41].Chadayammuri et al. Patient Safety in Surgery (2015) 9:32 Page 5 of 10

While preparing for graft harvest using the RIA
system, a few important considerations must be kept in
mind. The reaming head size should not exceed the
canal diameter at the isthmus of the femur (as deter-
mined on AP and lateral radiography) by more than 2
mm [42]. Reaming should be performed under fluoro-
scopic guidance using an alternating motion of advan-
cing and withdrawing at a slow enough pace to allow
proper irrigation and aspiration [42]. Once reaming is
complete, aspiration should be turned off to reduce
intraoperative blood loss.
Harvested bone graft should be loosely packed to
bridge the osseous defect. It is important to avoid tight
packing of bone graft when bridging the defect, as this
can precipitate necrosis of the graft due to impaired
angiogenesis. Large defects may require additional aug-
mentation of autogenous bone graft with allograft or
demineralized bone substitute at a ratio less than or
equal to 1:3 (autograft to allograft) to achieve sufficient
graft volume or strength [18, 19, 46]. Autogenous bone
graft may also be enhanced with synthetic bone morpho-
genetic protein (BMP) [47, 48], bisphosphonates [18], or
hydroxyapatite [18, 48]; however, the clinical utility of
such synthetic derivatives remains controversial. Indeed,
Masquelet et al observed increased autograft resorption
in patients receiving additional local injections of recom-
binant BMP-7 [19].
I. Postoperative course
Following surgery, the patient is encouraged to re-
sume immediate weight-bearing as tolerated. Early
weight-bearing stimulates secondary bone healing
(callus formation) and may help to reverse long-
standing physical and psychological disability. The
patient should be scheduled for routine follow-up
postoperatively to evaluate for fracture alignment, os-
seous consolidation, and functionality.
With careful planning and execution, reconstruction
of long bone osteomyelitis defects using Masquelet
technique can yield excellent long-term clinical out-
comes. In a case series of 25 patients presenting with 27
segmental bone loss nonunions averaging 5.8 cm in size
(range, 1 to 25 cm), 24 cases (90 %) demonstrated full
clinical and radiographic healing within 1 year following
reconstruction with Masquelet technique. No postopera-
tive complications, including infection recurrence, were
reported [49].
The technique described here to treat long bone osteo-
myelitis is a feasible option for most orthopaedic surgeons.
The materials required (PMMA cement, antibiotic pow-
der, D&C tubing and mineral oil) are readily available at
most centers. Carbon fiber products are becoming more
popular and readily available, although a standard metallic
nail can be substituted if necessary. Compared toalternative techniques such as bone transport and vascu-
larized fibular grafting, the Masquelet technique is often
technically easier and can produce good outcomes in a
majority of patients.
Case description
As an illustrative case example, we present a 33-year-old
male who sustained an open (Gustilo IIIB) diaphyseal frac-
ture of the right tibia following an occupational forklift
accident. Initial treatment was performed at an outside
facility and included multiple rounds of debridement
followed by open reduction and internal fixation (ORIF)
of a buttery fragment along the medial tibia with locking
plate and IM nailing. The patient was placed on wound
VAC therapy for three months and subsequently devel-
oped a chronic draining wound over the anterior tibia
with concomitant osteomyelitis (Fig. 1), prompting referral
to our Level I trauma center.
Upon initial presentation to our institution, the patient
was noted to have a foul-smelling wound with inflam-
matory hypergranulation surrounding an open 3 × 2 cm
bony defect. The patient reported a deep, throbbing pain
localized to the right anterior tibia with an intensity of
7/10 at rest. Past medical and family history were not
significant for any metabolic, endocrine, or chronic in-
flammatory conditions. The patient reported smoking
one-half pack of cigarettes daily for the past 10 years.
On physical examination, the patient had decreased sen-
sation to light touch over the cutaneous distribution of
the superficial peroneal nerve and had drastically reduced
strength of ankle dorsiflexion (1/5) in the right leg. Diag-
nostic imaging with anteroposterior (AP) and lateral ra-
diographs revealed a mid-diaphyseal comminuted fracture
nonunion of the right tibia with overlying soft-tissue
swelling (Fig. 2). Direct bone samples were obtained
Fig. 1 Preoperative clinical photograph demonstrating an anterior
wound with exposed boneChadayammuri et al. Patient Safety in Surgery (2015) 9:32 Page 6 of 10

for culture and pathology, which demonstrated a poly-
microbial infection comprised of Methicillin-resistant
Staphylococcus aureus (MRSA) and Streptococcus angino-
sus. The patient was started on a 6-week course of Vanco-
mycin (15 mg/kg IV q8 hr) and Metronidazole (500 mg
PO q8 hr).Given the patient ’s chronically infected nonunion and
extensive necrosis of surrounding soft-tissue, a two-staged
reconstruction using the Masquelet technique was per-
formed. First, plate and screws were removed. Intraopera-
tively, absence of pinpoint bleeding was noted along an 11
cm segment of devitalized bone. Under fluoroscopic guid-
ance, a Monotube multiplanar external fixator (Stryker,
Kalamazoo, Michigan) was placed. Tibial osteotomy was
carried out using an oscillating saw to resect necrotic
bone, followed by radical debridement of all nonviable
surrounding tissue. A PMMA cement spacer with 3.6 gm
tobramycin and 3 gm vancomycin was prepared and
placed into the bone defect. Soft-tissue coverage was pro-
vided using a rotational soleus flap. Incisional wound VAC
therapy was applied for 1 week to promote tissue granula-
tion and accelerated wound closure. The patient received
a 6-week course of Vancomycin and Flagyl after direct tis-
sue cultures grew MRSA andStreptococcus milleri .
Eight weeks post-operatively, the patient returned to
undergo the second stage of reconstruction. Intraoperative
Fig. 3 Intra-operative photograph of the self-induced periosteal
membrane during the second stage of reconstruction following
removal of the cement spacer. A cement coated antibiotic nail was
placed to provide bone stability and allow early weight bearing
Fig. 2 Preoperative lateral radiograph demonstrating a mid-diaphyseal
comminuted fracture of the right tibia and sequestrum (red arrow)
Fig. 4 Postoperative MRI of the tibia following definitive fixation
with radiolucent antibiotic-impregnated carbon-fiber IM nailing. Use
of the carbon-fiber IM nail enables artifact-free MRI visualizationChadayammuri et al. Patient Safety in Surgery (2015) 9:32 Page 7 of 10

bone and tissue samples were culture negative. A signifi-
cant self-induced periosteal envelope was visible overlying
the previously placed cement spacer (Fig. 3). A central in-
cision of the periosteal membrane was made in line with
the tibia. The cement spacer was removed and pins of the
external fixator were backed out until they were unicorti-
cal in nature. A 10-mm radiolucent carbon-fiber intrame-
dulary nail (Carbo-Fix, Collierville, TN, USA) was coated
with Palacos R cement (Warsaw, IN, USA) premixed with
3 g vancomycin and 3.6 g tobramycin. Under fluoroscopic
guidance, the nail was positioned in an anterograde man-
ner and locked with 2 proximal interlocking titanium
screws (Fig. 4). The ipilateral femur was used for autograft
harvest using RIA, which was loosely placed into the de-
fect. The soft tissues, including the periosteal membrane,
were closed in layers.
Patient outcome
The patient was followed at 2, 8, 12, and 20 weeks postop-
eratively. The patient was able to resume full weight-
bearing by 2 weeks postoperatively and was pain free at
his 3 month follow-up visit. Radiographs at his 5 month
visit showed consolidation of the defect without evidence
of infection, osteolysis, or hardware failure (Fig. 5).
Concluding remarks
✓The Masquelet technique is a viable option for
treatment of long bone PTOM. Primary advantages of
this technique include its length independence,
induction of a periosteal membrane that protects
against graft resorption, and eradication of infection
with an antibiotic-impregnated cement spacer that pre-
serves dead space volume for delayed reconstruction.✓Radical debridement should extend to viable bone
margins (as indicated by the paprika sign). Use of an
osteotome to perform corticotomy helps prevent
damage to healthy surrounding tissue.
✓Following thorough debridement, an antibiotic-
impregnated PMMA cement spacer is placed. Irrigation
with cold saline during preparation of the antibiotic-
cement mixture will help prevent skin burns.
✓Stabilization during the first stage can be achieved
with an external fixator, plate or IM nail.
✓The cement spacer must be left in place for 6-8 weeks.
Complete eradication of infection, confirmed by culture
and pathology, is a prerequisite to the second stage of the
procedure (reconstruction of the osseous defect).
✓In the second stage, we favor definitive fixation using
an antibiotic-coated carbon fiber IM nail. This allows
for artifact-free visualization on MRI, which is import-
ant for monitoring treatment response.
✓Autograft harvested using an RIA system should be
loosely packed around the IM nail to permit
angiogenesis. Bony margins should be freshened with a
drill bit to facilitate graft integration.
Consent
Obtained from patient.
Competing interests
Dr Cyril Mauffrey is Co investigator on a grant sponsored by Carbofix. No
other conflicts noted in relation to this manuscript.
Authors ’contribution
All authors contributed to this manuscript equally. The concept and
innovative idea came from CM. VC wrote most of the draft while critical
revisions and illustrations were done by CM and MH. Dr Cyril Mauffrey is Co
investigator on a grant from Carbofix. No other conflicts in relation to this
manuscript. All authors read and approved the final manuscript.
Fig. 5 Standard AP ( a) and lateral ( b) plain radiograph taken at 5 months postoperative follow-up demonstrating improved bone healing without
evidence of osteolysis, infection, or hardware migrationChadayammuri et al. Patient Safety in Surgery (2015) 9:32 Page 8 of 10

Declarations
The publication costs for this article were covered in full by a grant from the
Colorado Physician Insurance Company (www.copic.com) to Philip F. Stahel,
MD. COPIC had no influence on authorship or scientific content of this
article.
Author details
1University of Colorado School of Medicine, Aurora, CO, USA.2Department of
Orthopaedic Surgery, University of Michigan, Ann Arbor, MI, USA.
3Department of Orthopaedic Surgery, Denver Health Medical Center,
University of Colorado, School of Medicine, 777 Bannock Street, Denver, CO
80204, USA.
Received: 31 July 2015 Accepted: 5 October 2015
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