IN VITRO ACTIVITY OF BIOCOMPOSITES WITH ANTIBACTERIAL [611071]

IN VITRO ACTIVITY OF BIOCOMPOSITES WITH ANTIBACTERIAL
PROPRIETIES

CRISTINA RÎMBU, OANA BORHAN. ELEONORA GUGUIANU, CRISTINA HORHOGEA, CARMEN CRETU, MARIANA GRECU,
ANDREEA COZMA, ANGELA DĂNILĂ, AUGUSTIN MUREȘAN

Microbiology- -Immunology Laboratory , Department of Public Health , Faculty of Veterinary Medicine, University of Agricultural
Sciences and Veterinary Medicine Ion Ionescu de la Brad,Iași, Romania
2Department of Chemical Textile Finishing, Faculty of Textiles, Leather and Industrial Management, DzGheorghe Asachi dz Technical
University, Iasi, Romania,

•Introduction

Given the emergence of antibiotic resistance
and the recognized antimicrobial potential
of silver, we found it useful to test the
activity of silver nanoparticles incorporated
into textile structures in order to obtain
effective medical supplies against bacterial
strains with multiple resistance to common
antibiotics.

•Material and method

We have tested textiles (cotton)
samples treated by various technological
processes with silver nanoparticles.
The effect of the matrices treated with
AgNPs was tested against Staphylococcus
aureus ATCC 29213 and Pseudomonas
aeruginosa ATCC 27853 . Have also been
tested three strains (T1-T3) MRSA
(methicillin-resistant Staphylococcus aureus )
and three strains (T1-T3) Pseudomonas
aeruginosa ESBL ( extended-spectrum beta-
lactamases ) isolated from biological
samples taken from animals with otic and
dermal infections.
•Results and discussions

•Conclusions
•The best antimicrobial activity in vitro was
identified in the samples treated with silver
nanoparticles in the presence of polyacrylic
acid. The antibacterial effect was identified
on both standard strains of Staphylococcus
aureus ATCC 29213 and Pseudomonas
aeruginosa ATCC 27853 , as well as on the
three strains of MRSA and three strains of
Pseudomonas aeruginosa ESBL, isolated
from biological samples taken from
animals with various conditions.
• The susceptibility of these strains to the
silver nanoparticles action advocates the
applicative potential in the production of
textile materials for medical use.

USAMVB Timisoara
INTERNATIONAL CONFERENCE ON
LIFE SCIENCES
24-25 Mai 2018
Abstract : The study focused on the antimicrobial effect of cellulosic textile materials impregnated with silver nanoparticles. The purpose of these preliminary tests
was to identify and develop medical supplies with antimicrobial properties that can be used in veterinary and human medicine. Textile samples have different loading
levels with biologically active emulsions, and cellulosic materials impregnated with silver nanoparticles have been subjected to polyacrylic acid and glucose
treatments. In vitro testing was performed on Staphylococcus aureus ATCC 29213 , Pseudomonas aeruginosa ATCC 27853 and bacterial strains with antibiotic resistance
such as: three strains (T1-T3) MRSA ( methicillin-resistant Staphylococcus aureus ) and three strains (T1-T3) Pseudomonas aeruginosa ESBL ( extended-spectrum beta-
lactamases ), isolated from biological samples taken from animals with otic skin infections. The best antimicrobial activity was achieved in cellulosic matrices
impregnated with silver nanoparticles and treated with polyacrylic acid against Staphylococcus aureus and Pseudomonas aeruginosa . The increased sensitivity to
AgNPs of some antibiotic resistant strains is encouraging and is an argument for further research in this direction. Considering the day- to-day increase in the
resistance of bacteria to antibiotics, it is necessary to identify alternative therapeutic solutions.

Fig.3. Staph. aureus T1
sample Ag + polyacrylic acid
Fig.7. Ps.aeruginosa T1
sample Ag + polyacrylic acid