Obtinerea Unei Forme Proteice cu Actiune de Regenerare Tisulara Si cu Proprietati Antimicrobiene

Cuprins

Tema de disertație

Preambul

Capitolul I – Stadiul actual al cunoașterii

Capitolul II – Biopolimeri naturali. Structură și proprietăți fizico-mecanice

II.1. Biopolimeri de origine animală

II.1.1. Colagenul

II.1.2. Proteinele din albușul de ou

II.1.2.1. Ovalbumina

II.1.2.2. Ovotransferina

II.1.2.3. Lizozimul

II.2. Procedee de extracție și purificare a formelor proteice

II.2.1. Extracția și purificarea formelor colagenice

II.2.2. Obținerea și purificarea formelor proteice din albușul de ou

Capitolul III – Obținerea formelor proteice cu acțiune de regenerare tisulară și cu proprietăți antimicrobiene

III.1. Obținerea soluțiilor de colagen

III.2. Obținerea formelor proteice din albușul de ou

III.3. Caracterizarea electroforetică aformelor proteice obținute

Capitolul IV –Concluzii

Bibliografie

Bibliografie

A.Bigi,E.Boanini, B.Bracci, A. Facchini, S.Panzavolta, F.Segatti, L.Sturba, Nano-crystalline hydroxyapatite coatings on titanium:a new fast biomimetic method, Biomaterials 26, p.4085–4089, 2005.

I.Jones, L.Currie, R.Martin, A guide to biological skin substitutes, British Journal of Plastic Surgery 55, p.185-193, 2002.

H.Beele, Artificial skin: Past, present and future, The International Journal of Artificial Organs. 25(3), p.163-173, 2002.

E.Bell, B.Ivarsson, C.Merrill, Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro, Proc. Natl. Acad. Sci. USA. 76, p.1274-1278, 1979.

T.W.Wang, H.C.Wu, Y.C.Huang, J.S.Sun, F.H.Lin, Biomimetic Bilayered Gela-tin-Chondroitin 6 Sulfate-Hyaluronic Acid Biopolymer as a Scaffold for Skin Equivalent Tissue Engineering, Artificial Organs 30, p.141-149, 2006.

R.Chen, J.A.Hunt, Biomimetic materials processing for tissue-engineering pro-cesses, J.Mater.Chem. 17, p.3974 – 3979, 2007.

G.K.Sangamesh, P.N. Syam, J.Roshan, S.N.Lakshmi, T.L.Cato, Electrospun poly (lactic acid-co-glycolic acid) scaffolds for skin tissue engineering, Biomaterials 29, p.4100–4107, 2008.

S.Liao, Biomimetic electrospun nanofibers for tissue regeneration, Biomedical Ma-terials 1, p.45-53, 2006.

D.I.Zeugolis, S.T.Khew, E.S.Y.Yew, A.K.Ekaputra,Y.W.Tong, L.Y.L.Yung, D.W.Hutmacher, C.Sheppard, M.Raghunath, Electro-spinning of pure collagen nano-fibres – Just an expensive way to make gelatin?, Biomaterials 29, p.2293-2305, 2008.

M.Li, M.J.Mondrinos, M.R.Gandhi, F.K.Ko, A.S.Weiss, P.I.Lelkes, Electrospun protein fibers as matrices for tissue engineering, Biomaterials 26, p.5999-6008, 2005.

D.J.Cziperle, K.A.Joyce, C.W.Tattersall, Albumin impregnated vascular grafts: albumin resorption and tissue reactions, J Cardiovasc.Surg. 33, p 407- 414, 1992.

L.Xue, H.P.Greisler, Biomaterials in the development and future of vasculargrafts, J. Vascular Surgery 37, Issue 2, p. 472-480, 2003.

H.Yu, W.Dai, Z.Yang, P.Kirkman, F.A.Weaver, D.Eton, V.L.Rowe, Smooth muscle cells improve endothelial cell retention on polytetrafluoroethylenegrafts in vivo, J.Vasc Surg. 38(3), p.557–563, 2003.

S.F.Tao, L.Chen,Y.X.Zheng,Y.Xu, J.Chen, H.Yu, Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid, J.Zhejiang Univ.Sci.B. 7(6), p.421–428, 2006.

L.Kennedy, X.S.Wen, D.E.Carter, D.J.Abraham, A.Leask, Fibroblast adhesion results in the induction of a matrix remodeling gene expression program, Matrix Biology 27, Issue 4, p.274-281, 2008.

M. Colț, M.D. Bucevschi,„Valorificarea neconvențională a pieilor”, Editura „Gheorghe Asachi”, Iași, 1999.

F. Mano, G.A. Silva, H.S. Azevedo, P.B. Malafaya, et.al, J. R. Interface 4, 2007, 999-1030

Gh. Chiriță, M. Chiriță , Tratat de biomolecule, Editura Sedcom Libris, vol. 1, 2009.

J. Adzet, „Transformation of Lime Split Trimmings into Different Collagen Materials”, J.A.L.C.A., 105 (8),254-271, 2010.

G.Y.Li, S. Fukunaga, K. Takenouchi, „Physiological and Cell Biological Properties in vitro of Collagen Isolated from Calf Limed Splits”, J.S.L.T.C., Vol. 88 (2), 2004, 66-71.

P. Mokrejs, D. Janacova, P. Svoboda, „Modelling the solubility of films prepared from collagen Hydrolysate”, J.S.L.T.C., vol. 94 (6), 2010, 231-239.

Lydia Campbell, Vassilios Raikos, tephen R. Euston,”Modification of functional properties of egg-white proteins”, Molecular Nutrition, Food, Vol. 47 (6), 2003, 369-376.

http://www.rcsb.org/pdb/explore.do?structureId=1IEJ

DONOVAN, J.W.; MAPES, C.J. „A differential scanning calorimetric study of conversion of ovalbumin to s-ovalbumin”, Journal of the Science of Food and Agriculture, v.27, p.197-204, 1976.

DU, L.; PROKOP, A.; TANNER, R.D. „Effect of denaturation by preheating on the foam fractionation behavior of ovalbumin, Journal of Colloid and Interface Science, v.248, p.487-492, 2002.

M. Covaciu,F. Olaru, I. Petrescu, „Ovalbumin isoforms- purification and denaturation/renaturation studies”, Agricultură-Știință și practică, vol. 1-2, 61-62, 2007.

FEENEY, R.E. Egg proteins. In: SCHULTZ, H.W.; ANGLEMIER, A.F. (Ed.) Symposium on foods: proteins and their reactions. Wetsport: AVI Publishing, 1964.

FERRY, J.D. Protein gels, Advances in Protein Chemistry, vol. 4, 1948, 1- 78,.

Paul Messier, Protein Chemistry of Albumen Photographs „Topics in Photographic Preservation”, Vol. 4, 1991, 124-135.

A.Graszkiewicz, M.Zelazko, T.Triszka, „Application of pancreatic enzymes in hydrolysis of egg white proteins”, Pol. J. Food Nutr. Sci., vol 60 (1), 2010, 57-61.

R. Rohanizadeh, N. Kokabi, „Heat denatured/aggregated albumin-based biomaterial: effects of preparation parameters on biodegradability and mechanical properties”, J. Mater Sci. Mater Med., 2009, 20: 2413-2418.

F.F. Machado, J. S.R. Coimbra, E. E. Garcia Rojas, L. A. Minim, F. C. Oliviera, R. De Cassia S. Sousa, „Solubility and density of egg white proteins: Effect of pH and saline concentration”, Science Direct, LWT 40 (2007), 1304-1307.

Mireille Weijers, Fred van de Velde, Ann Stijnmam, Anne van de Pijpekamp, Ronald W. Visschers, „Structure and rheological properties of acid-inuced egg white protein gels”, Food Hydrocolloids, 20, 2006, 146-159.

Chi Yujie, Tian Bo, Sun Bo, Guo Mingruo, „Enzymatic Hydrolysis Conditions for Egg White Proteins”, Bull. Facul. Agric. Nugata Univ., 58(2), 2006, 143-146.

Yoon Jihee, Jae-Min park, Ki-Ju Kim, Yang-Hoon Kim, Jiho Min, „Antimicrobial activity of the cell organelles, lysosomes, isolated from egg white”, J. Microboil. Biotechnol., 19 (11), 2009, 1364-1368.

Theresa Y. Cho,Nolene Byrne, David J. Moore,Brian A. Pethica,C. Austen Angellb and Pablo G. Debenedetti, „Structure–energy relations in hen egg white lysozyme observed during refolding from a quenched unfolded statew”, Chem Commun, 2009, 4441-4443, www.rsc.org/chemcomm.

M.A.Williams, J.M.Thornton, J.M.Goodfellow „Modelling protein unfolding: hen egg-white lysozyme”, Protein Engineering vol.10 no.8, 1997, 895–903.

T. Kuberasampath și colab. – U. S. Pat. 6,468,308 (2002).

Breen, E. J., Hopwood, F. G., Williams, K. L., and Wilkins, M. R. (2000) Automatic poisson peak harvesting for high throughput protein identification. Electrophoresis 21, 2243 –2251[CrossRef][Medline].

Perkins, D. N., Pappin, D. J. C., Creasy, D. M., and Cottrell, J. S. (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551 –3567.

J.A. Bainj, H.F. Deutsch, „An electrophoretic study of the egg white proteins of varios birds”,download from www./jbc.org., 1947, 531-534.